LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1619..1715) /label=attL2 /note="attL2 (c)" misc_signal complement(1730..1749) /note="T7 Promoter/priming site (c)" primer_bind 1757..1773 /note="M13 Reverse priming site" gene 1886..2695 /note="Kanamycin resistance gene" rep_origin 2816..3489 /note="pUC origin" vector join(1627..3492,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1612 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9631 IMAGE:3913851" /tissue_type="Uterus, leiomyosarcoma" /clone_lib="NIH_MGC_71" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1612 /gene="A4GALT" /gene_synonym=A14GALT /gene_synonym=A4GALT1 /gene_synonym=P1 /gene_synonym=PK /db_xref="GeneID:53947" /db_xref="HGNC:18149" /db_xref="MIM:607922" CDS 692..1612 /dnas_title="alpha 1,4-galactosyltransferase" /gene="A4GALT" /gene_synonym=A14GALT /gene_synonym=A4GALT1 /gene_synonym=P1 /gene_synonym=PK /codon_start=1 /product="alpha 1,4-galactosyltransferase" /protein_id="AAH17068.1" /db_xref="GI:16877647" /db_xref="GeneID:53947" /db_xref="HGNC:18149" /db_xref="MIM:607922" /translation="MSKPPDLLLRLLRGAPRQRVCTLFIIGFKFTFFVSIMIYWHVVG EPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAA RTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYA AVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNG AFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGV TTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALL AQLHARYCPTTHEAMKMYL" misc_feature 692..1612 /note="A4GALT coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaggagaaa gggcagctct ataacctgcc 721 agcagagatc ccctgcccca ccttgacacc ccccacccca ccctcccacg gccccactcc 781 aggcaacatc ttcttcctgg agacttcaga ccggaccaac cccaacttcc tgttcatgtg 841 ctcggtggag tcggccgcca gaactcaccc cgaatcccac gtgctggtcc tgatgaaagg 901 gcttccgggt ggcaacgcct ctctgccccg gcacctgggc atctcacttc tgagctgctt 961 cccgaatgtc cagatgctcc cgctggacct gcgggagctg ttccgggaca cacccctggc 1021 cgactggtac gcggccgtgc aggggcgctg ggagccctac ctgctgcccg tgctctccga 1081 cgcctccagg atcgcactca tgtggaagtt cggcggcatc tacctggaca cggacttcat 1141 tgttctcaag aacctgcgga acctgaccaa cgtgctgggc acccagtccc gctacgtcct 1201 caacggcgcg ttcctggcct tcgagcgccg gcacgagttc atggcgctgt gcatgcggga 1261 cttcgtggac cactacaacg gctggatctg gggtcaccag ggcccgcagc tgctcacgcg 1321 ggtcttcaag aagtggtgtt ccatccgcag cctggccgag agccgcgcct gccgcggcgt 1381 caccaccctg ccccctgagg ccttctaccc catcccctgg caggactgga agaagtactt 1441 tgaggacatc aaccccgagg agctgccgcg gctgctcagt gccacctatg ctgtccacgt 1501 gtggaacaag aagagccagg gcacgcggtt cgaggccacg tccagggcac tgctggccca 1561 gctgcatgcc cgctactgcc ccacgacgca cgaggccatg aaaatgtact tgTAGGACCC 1621 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 1681 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 1741 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 1801 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 1861 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 1921 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 1981 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2041 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2101 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2161 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2221 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2281 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2341 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 2401 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 2461 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 2521 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 2581 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 2641 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 2701 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 2761 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 2821 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 2881 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 2941 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3001 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3061 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3121 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3181 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3241 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3301 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3361 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 3421 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 3481 TGCTCACATG TT //